mouse ccl2 elisa kit  (R&D Systems)

Journal: The FASEB Journal

Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight

doi: 10.1096/fj.202600151RR

Figure Lengend Snippet: Vagotomy promoted Ly6G + cell infiltration into eWAT. Wild‐type mice were subjected to left cervical vagotomy (VX) or sham surgery and tissues were collected at 7 days following surgery. (A) The percentage of non‐adipocyte nuclei of total nuclei per section was quantified using ImageJ ( n = 4) and right panels show representative images of paraffin sections of eWAT stained with H&E in sham and VX animals. (B) CCL2 release from eWAT was analyzed by ELISA. The bar shows the CCL2 levels from sham ( n = 4) or VX ( n = 4) mice normalized to eWAT weight: ng/mL per g ± SEM (unpaired Student's t test). (C) eWAT was collected at 1 ( n = 3), 4 ( n = 4 sham, n = 5 VX), and 7 ( n = 15) days following VX or sham surgery and the eWAT SVCs were analyzed by flow cytometry. The bar shows the % ± SEM of CD11b + Ly6G + cells from CD45 + (one‐way ANOVA, Uncorrected Fisher's LSD). (D) Graphs show representative gating for CD11b + Ly6G + cells in sham and VX eWAT at 7 days (concatenated n = 5–6). (E) Representative immunostaining of Ly6G (red) and Perilipin1 (green) in paraffin sections of eWAT. (F–H) Bone marrow neutrophils after sham ( n = 9) or VX ( n = 5) surgery were isolated using negative magnetic beads and analyzed using bulk RNAseq (DESeq2). Heatmap (F), volcano plot (G) of differentially expressed genes, and GO (Gene Ontology) (H) enrichment bar plot. ns = not significant, * p < 0.05. VX, Vagotomy; eWAT, epididymal white adipose tissue; H&E, hematoxylin–eosin; SVCs, stromal vascular cells.

Article Snippet: In vitro release rate was calculated as mg NEFAs per mg eWAT tissue per hour. (2) CCL2 levels were quantified using a mouse CCL2 ELISA kit (R&D Systems, #DY497‐05), according to the manufacturer's instructions ( n = 1 experiment).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunostaining, Isolation, Magnetic Beads, RNA sequencing

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet: Intraperitoneal administration of V7-LEP/ADIPOQ vector rescues the excessive weight gain and impaired glycemic control in ob/ob mice (A) Schematic of the AAV vector containing human leptin and human adiponectin linking by 2A sequence. (B) Body weight. (C) Area under the curve of body weight. (D) Weight gain. (E) Area under the curve of weight gain. (F) Glucose tolerance test at 8-week post AAV injection. (G) Area under the curve of glucose tolerance test. Data are means ± SEM. Sample size (biological replicates): ob /+ buffer n = 5, ob/ob GFP 8E10 n = 5, ob/ob LEP/ADIPOQ 4E10 n = 4, ob/ob LEP/ADIPOQ 8E10 n = 4. ∗ p < 0.05, ∗∗ p < 0.01 from one-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Mouse Leptin ELISA , R&D System , Cat#DY498.

Techniques: Plasmid Preparation, Control, Sequencing, Injection, Comparison

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet: Intraperitoneal administration of V7-LEP/ADIPOQ reverses hyperinsulinemia, hyperglycemia, and hepatic steatosis in ob/ob mice (A) Serum levels of human leptin and human adiponectin. No significant difference between the low dose and high dose. (B) Serum level of insulin. (C) Serum level of glucose. (D) Liver triglyceride content. Data are means ± SEM. Sample size (biological replicates): ob /+ buffer n = 5, ob/ob GFP 8E10 n = 5, ob/ob LEP/ADIPOQ 4E10 n = 4, ob/ob LEP/ADIPOQ 8E10 n = 4. ∗ p < 0.05, ∗∗ p < 0.01 from One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Mouse Leptin ELISA , R&D System , Cat#DY498.

Techniques: Comparison

Journal: iScience

Article Title: Development of an adipose-tropic AAV capsid ablating liver tropism

doi: 10.1016/j.isci.2024.110930

Figure Lengend Snippet:

Article Snippet: Mouse Leptin ELISA , R&D System , Cat#DY498.

Techniques: Recombinant, SYBR Green Assay, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Mutagenesis, Reverse Transcription, Software

Journal: bioRxiv

Article Title: Early body weight gain in TALLYHO/JngJ mice predicts adult diabetic phenotype, mimicking childhood obesity

doi: 10.1101/2024.01.29.577705

Figure Lengend Snippet: Hyperphagia and polydipsia in diabetic male TallyHo mice. Food (A) and water (B) consumption of male TallyHo mice at 6 and 18 weeks of age measured in metabolic cages. Measurement was performed on two consecutive days for each 21 hours. Data presented are mean values of both measurements. *** indicate p<0.001 in the Mann-Whitney test, horizontal lines indicate mean values. Blood leptin concentrations in male TallyHo mice at 8 weeks (C) and 20 weeks (D) of age. Line indicates linear regression; r is the Pearson correlation coefficient (8 weeks: r = 0.3910, 20 weeks: r = 0.7867).

Article Snippet: ELISA: Insulin ELISA (Mouse Insulin ELISA, Mercodia) and Leptin ELISA (Quantikine Mouse/Rat Leptin Immunoassay, R&D Systems) followed manufacturer’s protocols.

Techniques: MANN-WHITNEY